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In molecular biology, small interfering RNAs (siRNAs) have emerged as pivotal tools for regulating gene expression through RNA interference (RNAi). These double-stranded RNA molecules, consisting of 19-23 base pairs, play a crucial role in research and drug development by inducing the degradation of target mRNA and inhibiting gene expression.This article explores the complex world of siRNA drugs, highlighting their status in the market and their expanding role in clinical research. It explores the early in vivo pharmacokinetic (PK) studies of siRNAs, underscoring the necessity for diverse bioanalytical platforms. This diversity is essential due to the complex behavior of siRNAs in various biological environments. The article examines critical aspects of siRNA analysis, including exposure levels, tissue distribution, the concentration of RNA-induced silencing complexes, and the effectiveness in deg…